Skip to main content
Figure 1 | Breast Cancer Research

Figure 1

From: Bone marrow-derived, alternatively activated macrophages enhance solid tumor growth and lung metastasis of mammary carcinoma cells in a Balb/C mouse orthotopic model

Figure 1

M2-polarized macrophages enhance tumor growth and increase cell proliferation indices. 4T1-luc cells and/or M2-polarized macrophages (M2-Mϕs) were injected into the mammary fat pads of female Balb/C mice. Bioluminescent signals were monitored on the days indicated. (A) Representative images of luciferase signals. (B) Quantitative analysis of luminescence. Each bar represents the mean ± standard error of the mean (SEM) (n = 8). (C) Immunohistochemical (IHC) staining for Ki-67, cyclin-dependent kinase (CDK)2, and CDK4 in tumor sections was based on 3,3'-diaminobenzidine staining, as described in the Materials and methods section. (D) 4T1-luc cells and/or M2-Mϕs were cultured in 24-well plates. Bioluminescent signals were monitored at the indicated day. Each bar represents the mean ± SEM from three independent experiments performed in duplicate. *Significantly different from the 4T1 group, P < 0.05.

Back to article page

Breast Cancer Research

ISSN: 1465-542X

Contact us