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Figure 1 | Breast Cancer Research

Figure 1

From: The intronic G13964C variant in p53 is not a high-risk mutation in familial breast cancer in Australia

Figure 1

Detection of the G13964C variant by PCR-RFLP assay. The C→G nucleotide substitution creates a HhaI site, and the G allele is detected by HhaI digestion of the 131-bp product to 98-bp and 33-bp products (the 33-bp product is not adequately resolved on this gel). Lanes 1 and 2, GG; lane 3, CG; lane 4, CC; lanes 5 and 6, no template control; and lane 7, BRL 1-kb ladder size marker.

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