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Figure 5 | Breast Cancer Research

Figure 5

From: Crk adaptor proteins act as key signaling integrators for breast tumorigenesis

Figure 5

Crk knockdown alters p130Cas phosphorylation. Control and Crk shRNA MDA-231 1833TR cells plated on fibronectin one hour before fixation; stained for phospho-p130Cas and phalloidin. Phospho-p130Cas is prominent at membrane ruffles in control cells (white arrowheads), but cytosolic in Crk shRNA cells, with decreased accumulation of phalloidin at cell periphery (white arrowheads). Scale bars 10 μm (A). Western blot of proteins from control and Crk shRNA MDA-231 1833TR cells plated for 0.5 hour on fibronectin were quantified using Odyssey software. Phospho-p130Cas levels were normalized to total p130Cas levels (n = 3) (B). Immunofluorescence of CrkI/II and phosphorylated p130Cas was performed on sections from control and Crk shRNA MDA-231 1833TR samples three days post-injection. Scale bars 20 μm (C).

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