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Figure 4 | Breast Cancer Research

Figure 4

From: Crk adaptor proteins act as key signaling integrators for breast tumorigenesis

Figure 4

Crk proteins are essential for outgrowth in the primary site. Mammary tumor outgrowth was measured and the average volume of control (n = 10 per cell line) and Crk shRNA tumors (n = 8 per cell line) plotted over time +/- SEM (A). H & E (5x) and Ki67 (20x) staining were performed on sections from control, Crk shRNA and CrkI/II rescue samples three days post-injection. Scale bars 50 μm. Average cell proliferation +/- SEM was quantified using ImageScope (B). Representative H&E staining (2x) of lesion size eight days post-injection are shown. Scale bars 10 μm. Average lesion size eight days post-injection was plotted +/- SEM (C). Soft agar growth of control, Crk shRNA and CrkI/II rescue 1833TR cell lines; mean +/- SEM. Scale bars 100 μm (D). Soft agar growth of control and Crk shRNA SUM1315 cell lines; mean +/- SEM. Scale bars 100 μm. Soft agar quantified using Infinity Analyze (E). Ki67 (20x) staining of sections from control and Crk shRNA SUM1315 tumor samples three days post-injection. Scale bars 50 μm. Average cell proliferation +/- SEM quantified using ImageScope (F). Statistically significant data illustrated as (* = P < 0.05, ** = P < 0.01, *** = P < 0.001).

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