Figure 4From: Crk adaptor proteins act as key signaling integrators for breast tumorigenesisCrk proteins are essential for outgrowth in the primary site. Mammary tumor outgrowth was measured and the average volume of control (n = 10 per cell line) and Crk shRNA tumors (n = 8 per cell line) plotted over time +/- SEM (A). H & E (5x) and Ki67 (20x) staining were performed on sections from control, Crk shRNA and CrkI/II rescue samples three days post-injection. Scale bars 50 μm. Average cell proliferation +/- SEM was quantified using ImageScope (B). Representative H&E staining (2x) of lesion size eight days post-injection are shown. Scale bars 10 μm. Average lesion size eight days post-injection was plotted +/- SEM (C). Soft agar growth of control, Crk shRNA and CrkI/II rescue 1833TR cell lines; mean +/- SEM. Scale bars 100 μm (D). Soft agar growth of control and Crk shRNA SUM1315 cell lines; mean +/- SEM. Scale bars 100 μm. Soft agar quantified using Infinity Analyze (E). Ki67 (20x) staining of sections from control and Crk shRNA SUM1315 tumor samples three days post-injection. Scale bars 50 μm. Average cell proliferation +/- SEM quantified using ImageScope (F). Statistically significant data illustrated as (* = P < 0.05, ** = P < 0.01, *** = P < 0.001).Back to article page