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Figure 3 | Breast Cancer Research

Figure 3

From: Crk adaptor proteins act as key signaling integrators for breast tumorigenesis

Figure 3

Crk proteins are required for efficient breast cancer outgrowth in bone. Outgrowth of 1833TR control (n = 7 for control 1, n = 4 for control 2) or Crk shRNA cells (n = 6 for Crk shRNA1, n = 6 for Crk shRNA 2) was measured by bioluminescence. Representative luciferase images at Day 40 are shown, closely resembling the mean bioluminescence values (A). Bioluminescence of positive limbs was quantified as relative luciferase units (RLU) over time. Averages from each cell line were plotted on a log scale +/- SEM (B). Representative X-ray images from negative control, control 1833TR and Crk shRNA mice. The average number and size of osteolytic lesions was blindly scored. Both control (n = 8) and Crk knockdown cohorts (n = 6) that exhibited osteolytic lesions were pooled for analysis (C). Statistically significant data illustrated as (* = P < 0.05, ** = P < 0.01, *** = P < 0.001).

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