Figure 2

Knockdown of Crk alters migration and invasion, cell morphology and adhesion. Western blot of Crk proteins from whole cell lysates with actin as loading control (A). Alamar blue assay (n = 3) of control and Crk shRNA cells (B). Control and Crk shRNA cells were analyzed for migration (C) and invasion (D) using transwells (n = 3). Control and Crk shRNA cells plated on fibronectin coated coverslips (63x). Control cells exhibit lamellipodia (white arrowheads) compared to Crk shRNA cells, which lack these structures (white arrowheads). Cells co-stained with anti-paxillin and phalloidin. Scale bars represent 10 μm (E). Cell spreading (n = 3) was quantified by counting the total number of spread cells versus total cell number (F). Error bars represent SEM. Statistically significant data illustrated as (* = P < 0.05, ** = P < 0.01, *** = P < 0.001).