CHD5 inhibits the growth of breast cancer cells both in vitro and in nude mice. (A) Confirmation of CHD5 mRNA expression by semi-quantification RT-PCR in parental MDA-MB-231 cells, stable vector control clones (V1 and V2), and stable population (Smix) and clones (S10 and S14) of CHD5-transfected cells. GAPDH was used as an internal control. (B) Expression of CHD5 inhibits cell proliferation in MDA-MB-231 cells, as examined by SRB staining. (C, D) Expression of CHD5 represses tumor growth in nude mice, as indicated by tumor volumes (C) and tumor weights (D) (8 mice were used for each group).