Figure 3
From: Chromodomain helicase DNA binding protein 5 plays a tumor suppressor role in human breast cancer
Immunohistochemical detection of CHD5 protein in normal tissues and primary tumors of the human breast. (A) Validation of CHD5 antibody by detecting CHD5 protein in CHD5-positive T-47D cells but not in CHD5-negative ZR-75-1 cells by IHC with the CHD5 antibody. Rabbit preimmune IgG was used as the negative control. (B, C) Normal breast tissue stained with rabbit anti-CHD5 antibody (B) or preimmune rabbit IgG (C, negative control). Arrows indicate cells that are positive for CHD5 staining. (D-G) Representative breast cancers showing no CHD5 protein expression (D) and low (E), medium (F) and high (G) levels of CHD5 expression.