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Figure 5 | Breast Cancer Research

Figure 5

From: CIP2A is a target of bortezomib in human triple negative breast cancer cells

Figure 5

In vivo effect of bortezomib on human breast cancer cell lines xenograft nude mice. A, Bortezomib decreased the size of HCC 1937 tumors (left), but had no anti-tumor effects on MCF-7 tumors (right). Points, mean (n = 6); bars, SE; * P < 0.05. B, Bortezomib reduced the weight of HCC 1937 tumors (left), but had no effect on that of MCF-7 tumors (right). Columns, mean (n = 6); bars, SD; * P < 0.05. C, Western blot analysis of CIP2A, p-Akt, Akt, and PARP cleavage in HCC 1937 and MCF-7 tumors. In vivo evidence of apoptosis is shown by PARP cleavage in HCC1937 tumors. D, Body weight of xenograft mice bearing HCC 1937 tumors (left) and MCF-7 tumors (right) during the in vivo experiment. Points, mean (n = 6); bars, SE. E, Bortezomib inhibited tumor growth of another two triple negative breast tumor xenografts (MDA-MB-231 and MDA-MB-468). Points, mean (n = 6); bars, SE; * P < 0.05. Male NCr athymic nude mice (five to seven weeks of age) were used for experiments (A) to (D), and female athymic nude mice were used for experiment (E). Mice were treated with intra-peritoneal injections of bortezomib (1 mg/kg body weight) twice weekly. Controls received vehicle. CIP2A, cancerous inhibitor of PP2A; HCC, hepatocellular carcinoma; PARP, poly ADP-ribose polymerase; SD, standard deviation; SE, standard error.

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