Figure 2

NG2 expression in human mammary tumor stroma. Sections of non-triple negative human ductal adendocarcinoma were immunolabeled to demonstrate NG2 expression by elements of the tumor stroma. (A-C) Sections were labeled for CD31 (A, blue) and NG2 (B, red). C, merged images from boxed area in B, showing close apposition of pericytes and endothelial cells. (D-F) Sections were labeled for αSMA (D, green) and NG2 (E, red). F, merged images from boxed area in E. Arrowheads indicate overlap between NG2 and αSMA labeling. (G-I) Sections were labeled for CD11b (G, green) and NG2 (H, red). I, merged images from boxed area in H. Arrowheads indicate overlap between NG2 and CD11b labeling. (J-L) Sections were labeled for CD31 (J, blue) and NG2 (K, red). L, merged images from boxed area in K). Images shown are representative of six ductal adenocarcinoma specimens that we examined. Scale bars = 25 μm in C, F, I, L. In all other panels, scale bars = 100 μm. Specimens 1, 3, and 8 in Table 1 were used to obtain these images. NG2, nerve-glial antigen 2; αSMA, α smooth muscle actin.