Skip to main content
Figure 3 | Breast Cancer Research

Figure 3

From: Deficiency of the p53/p63 target Perp alters mammary gland homeostasis and promotes cancer

Figure 3

Perp deficiency perturbs mammary epithelial homeostasis. (A) Western blot analysis of a variety of desmosomal proteins in wild-type and Perp-null mammary epithelial cells. GAPDH serves as a loading control. Dsg, desmoglein; Dp, desmoplakin; Pg, plakoglobin, Dsc, desmocollin. (B) Schematic diagram detailing the process of mammary transplantation, adapted from Edwards et al. [51]. (C) Carmine alum-stained whole-mount preparations of mammary epithelia at different stages. Cleared mammary fat pads were transplanted with wild-type or Perp-null mammary epithelium and allowed to develop to the indicated stages: Virgin, 8 weeks old; early pregnancy, day 3.5 after coitus; late pregnancy, day 14.5 after coitus; lactation, day 2 after parturition; involution, day 5 after weaning. (D) Whole-mount preparations (top row), hematoxylin and eosin (H&E)-stained sections (middle row), and CD45-stained section (bottom row) of 8-week-old virgin mouse mammary glands transplanted with wild-type or Perp-deficient mammary epithelium, showing lymphocytic aggregates (arrows) and mammary ducts (arrowheads). H&E and CD45-stained images were photographed at 100× and 400× magnification, respectively. (E) The difference between the numbers of foci in wild-type and Perp-deficient mammary whole mounts is graphically depicted. Statistical analysis was performed by using the Mann-Whitney test, for which * indicates statistical significance, P = 0.0286, n = 4 for each group.

Back to article page