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Figure 1 | Breast Cancer Research

Figure 1

From: Deficiency of the p53/p63 target Perp alters mammary gland homeostasis and promotes cancer

Figure 1

Perp is expressed in the mammary epithelium. (A) Upper left, Representative hematoxylin and eosin (H&E)-stained cross section of a wild-type virgin mouse mammary duct to demonstrate morphology. Lower left, Perp immunofluorescence of wild-type virgin mouse mammary gland sections, preincubated without (-Peptide) or Lower right, with the peptide (+Peptide) used to generate the Perp antibody, to demonstrate specificity of antibody staining. Arrows indicate potential Perp staining in myoepithelial cells. Upper right, A Perp-/- mammary gland section stained for Perp is also shown as a control. (B) Perp immunofluorescence image of stained cultured wild-type primary mammary epithelial cells. (C) Western blot analysis of wild-type and Perp-null mammary epithelial cells (MECs) and keratinocytes (ker) showing levels of Perp. GAPDH serves as a loading control. (D) Schematic diagram of a mammary duct in cross section, adapted from Adriance et al. [8]. (E) Dual-labeled immunofluorescence of wild-type virgin mouse mammary gland sections detailing localization of Perp and markers of the myoepithelium and luminal epithelium, smooth muscle actin (SMA), and keratin 8 (K8), respectively. Nuclei are marked with DAPI staining, and composite images are shown in merged panels. Images are taken at ×630 magnification.

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