In vivo analysis of transcription factor occupancy at cis -regulatory sites. The data show occupancy fold enrichment compared to chromatin input, and corrected against a negative control. The genotype of the cell lines used for each experiment is shown in the legend. (A) PCR analysis for ChIP of OCT-1 and C/EBPα at the rs1799943 site. (B) PCR analysis for ChIP of HMGA1 at the rs9567576 site. Sequencing chromatograms showing preferential pull-down of T allele in the immunoprecipitate in comparison with input chromatin in a heterozygous cell line. (C) PCR analysis for ChIP of DBP and ZF5 at the rs4942485 site. The data are the average of three replicates ± SD in two different cell lines. ChIP, chromatin immunoprecipitation; DBP, d-binding protein; PCR, polymerase chain reaction; SD, standard deviation.