Impact of RNA interference silencing of genes mapping to the 19q12 amplicon on cell viability. Bar plots of the survival fraction relative to non-targeting siRNA control of breast cancer cells with (red) or without (black) 19q12 amplification upon transfection with short interfering RNA (siRNA) SMARTpools targeting UQCRFS1, VSTM2B, POP4, PLEKHF1, C19ORF12, CCNE1, C19ORF2, ZNF536 and TSHZ3. Cell viability was assessed after 10 days with CellTiter-Glo® cell viability assay. P-values of t-test of the survival fraction between cancer cells harbouring the amplification of the gene tested and those lacking its amplification (P < 0.05 was considered significant). Error bars represent the standard error of the mean of three replicates.