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Figure 2 | Breast Cancer Research

Figure 2

From: Insulin-like growth factor 1 attenuates antiestrogen- and antiprogestin-induced apoptosis in ER+ breast cancer cells by MEK1 regulation of the BH3-only pro-apoptotic protein Bim

Figure 2

IGF-1 attenuates 4-OHT and/or MIF-induced cell death by reducing ROS levels. (a, b) ROS levels in cells undergoing hormonal treatments in the presence or absence of IGF-1 (a) or vitamin E (b). (c) The percentage of mitochondrial membrane permeabilization in cells undergoing hormonal treatments in the presence or absence of vitamin E. (d) The levels of cleaved PARP and lamin A in cells treated with 4-OHT and/or MIF in the presence and absence of vitamin E. fold diff., the levels of cleaved PARP or lamin A relative to levels in E2-treated cells, which were arbitrarily assigned a value of 1.0; β-actin served as a loading control. (a-d) MCF-7 cells treated with E2, E2 + 4-OHT, E2 + MIF, E2 + 4-OHT + MIF in the presence or absence of IGF-1 (20 ng/ml) or vitamin E (500 μ;M) for the indicated times were harvested for determination of ROS levels, percentage mitochondrial membrane permeabilization, or levels of cleaved PARP and laminA, as described in Materials and Methods. Values are expressed as mean ± SD (n = 3). Statistically significant differences are identified between comparisons of the following treatment groups: (a) E2 versus E2 + vitamin E; (b) E2 + 4-OHT versus E2 + 4-OHT+ IGF-1 or E2 + 4-OHT + vitamin E; (c) E2 +MIF versus E2 + MIF+IGF-1 or E2 + MIF+ vitamin E; and (d) E2 + 4-OHT + MIF versus E2+ 4-OHT + MIF + IGF-1 or E2 + 4-OHT + MIF + vitamin E. *P < 0.001.

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