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Figure 4 | Breast Cancer Research

Figure 4

From: Inhibition of phosphatidylcholine-specific phospholipase C results in loss of mesenchymal traits in metastatic breast cancer cells

Figure 4

Effects of D609 on the expression of vimentin and other differentiation markers in MDA-MB-231 cells. (a) Vimentin expression detected by Western blot in MDA-MB-231 and MCF-10A cells (actin, loading control). The expression and distribution of vimentin (red) were evaluated by confocal laser scanning microscopy (three-dimensional reconstruction images) in D609-treated or untreated (CTR) MDA-MB-231 cells. Lipid body formation was monitored by Bodipy 493/503 staining (green). Nuclei are reported in blue (DAPI). Scale bar, 20 μm. (b, c) Changes in protein expression of N-cadherin, E-cadherin, PC-PLC, MFG-E8, and galectin-3, evaluated by Western blot analyses of MDA-MB-231 cells incubated with D609 (actin, loading control). Experiments in panels (a-c) were independently repeated three times with similar results. CTR, control; DAPI, 4',6-diamidino-2-phenylindole; PC-PLC, phosphatidylcholine-specific phospholipase C.

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Breast Cancer Research

ISSN: 1465-542X

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