Figure 4

Western blot analysis confirms protein changes in MCF-7-TamR cells initially identified in quantitative proteomics. A) A representative Western blot of control (con) and tamoxifen resistant (MCF-7-TamR) cells showing the different expression levels of the indicated proteins. B) Four independent samples from each cell line were used to quantify the levels of each protein. The housekeeping protein Rho-GDI was used as an internal loading control. For comparative purposes, the control samples were set to 100%.