Figure 1

HSulf-2 knockdown attenuates tumor growth. (A) Western immunoblot analysis using anti-HSulf-2 and anti-tubulin antibodies shows efficient down-regulation of HSulf-2 in HW11 and 13 compared to NTC cells. (B) NTC and HSulf-2 depleted clones were injected in mouse mammary fat pad as described in Materials and methods. Tumor growth was monitored at Weeks 3, 5 and 7. Eight mice per group were evaluated. Inset: Excised tumors from NTC and HSulf-2 depleted clones. Graphs show quantitation of the tumor volume at indicated intervals among the groups. P-value < 0.05 (Student t test analysis).