Figure 2From: Immunohistochemical detection of Polo-like kinase-1 (PLK1) in primary breast cancer is associated with TP53mutation and poor clinical outcomeAnti-PLK1 antibody 208G4 detects only PLK1 in extracts of several breast cancer-derived cell lines. Cell extracts were prepared by lysing the cells in SDS-sample buffer and the proteins were subsequently resolved by SDS-PAGE. Western blotting analysis reveals a single band of molecular weight 62 kD corresponding to full length PLK1 in each of the cell extracts.Back to article page