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Figure 6 | Breast Cancer Research

Figure 6

From: Focal adhesion kinase contributes to proliferative potential of ErbB2 mammary tumour cells but is dispensable for ErbB2 mammary tumour induction in vivo

Figure 6

Focal adhesion kinase FAK is dispensable for ErbB2 mammary tumour metastasis. (a) FAK and Neu expression in FAKwt/wt (left) and FAKflox/flox (right)/mouse mammary tumour virus (MMTV)-NIC end-stage mammary tumours was determined by immunoblotting. Cytokeratin 8 (CK8) was used as a control for epithelial content. β-actin was used as a loading control. Data are representative of at least 10 animals from each genotype. (b) Immunohistochemistry analyses of Cre (upper) and FAK (lower) expression on paraffin sections from FAKflox/flox/MMTV-NIC end-stage mammary tumours. FAK expression is detectable only in the stromal compartment, whereas normal mammary ducts are Cre-positive and FAK-negative. Scale bars: 50 μm. (c) Bar graphs indicate the percentage of FAKwt/wt, FAKflox/wt and FAKflox/flox/MMTV-NIC mice that developed lung metastases. (d) Bar graphs indicate the number of metastatic foci per lung lobe in metastasis-positive animals. Error bars represent standard error of the mean (SEM). No statistically significant differences were observed (Student's t-test).

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