Figure 6From: Contribution of CXCL12 secretion to invasion of breast cancer cellsRat mammary adenocarcinoma CXCL12 cells show increased invasion to epidermal growth factor and recruitment of macrophages. (A) The in vivo invasion assay was performed with MTLn3 empty vector and CXCL12-overexpressing cell line tumors, allowing tumor cells to invade over 4 hours into microneedles containing 25 nM EGF or buffer. Data shown are means and SEM. *P < 0.05 and ***P < 0.0005 by t-test. (B) F4/80 staining was quantified by using a 40× lens objective and counting the number of F4/80-stained macrophages per field (n = 5 tumors per cell line and 10 random fields per tumor). Data shown are means and SEM. **P < 0.005 by t-test. (C) Representative images of F4/80 staining for macrophages in MTLn3 pQCXIP and MTLn3 pQCXIP-CXCL12 tumors. Images were taken using a 40× lens objective. Scale bar = 25 μm.Back to article page