Small interfering RNA library screen identified polo-like kinase-1 (PLK1) as a potential therapeutic target for breast cancer that uniquely eliminates tumor-initiating cells

Hu, Kaiji; Law, Jennifer H; Fotovati, Abbas; Dunn, Sandra E

doi:10.1186/bcr3107

Table 1 Partial list of the active kinases identified in the siRNA library screen

From: Small interfering RNA library screen identified polo-like kinase-1 (PLK1) as a potential therapeutic target for breast cancer that uniquely eliminates tumor-initiating cells

Accession number	Symbol	Brief description	Growth inhibition (%)
NM-001786	CDC2	Cell-division cycle 2, G₁ to S and G₂ to M	68^a
NM-033487	CDC2L1	Cell-division cycle 2-like 1 (PITSLRE proteins)	71
NM-001274	CHEK1	CHK1 checkpoint homolog (S. pombe)	61
NM-001896	CSNK2A2	Casein kinase 2, alpha prime polypeptide	62^a
NM-001929	DGUOK	Deoxyguanosine kinase	61
NM-000162	GCK	Glucokinase (hexokinase 4, maturity-onset diabetes of the young 2)	62
NM-153273	IHPK1	Inositol hexaphosphate kinase 1	55
NM-001569	IRAK1	Interleukin-1 receptor-associated kinase 1	59
XM-498294	LOC392265	Similar to cell-division protein kinase 5 (tau protein kinase II catalytic subunit) (TPKII catalytic subunit) (serine/threonine-protein kinase PSSALRE)	67
NM-005922	MAP3K4	Mitogen-activated protein kinase kinase kinase 4	57
NM-015133	MAPK8IP3	Mitogen-activated protein kinase 8 interacting protein 3	56
NM-006206	PDGFRA	Platelet-derived growth factor receptor, alpha polypeptide	63
NM-006212	PFKFB2	6-Phosphofructo-2-kinase/fructose-2,6-biphosphatase 2	60
NM-012395	PFTK1	PFTAIRE protein kinase 1	53
NM-000294	PHKG2	Phosphorylase kinase, gamma 2 (testis)	65
NM-018323	PI4K2B	Phosphatidylinositol 4-kinase type-II beta	51^a
NM-004570	PIK3C2G	Phosphoinositide-3-kinase, class 2, gamma polypeptide	69^a
NM-002651	PIK4CB	Phosphatidylinositol 4-kinase, catalytic, beta polypeptide	56
NM-181805	PKIG	Protein kinase (cAMP-dependent, catalytic) inhibitor gamma	65
NM-002658	PLAU	Plasminogen activator, urokinase	57
NM-005030	PLK1	Polo-like kinase 1 (Drosophila)	86^a
NM-006254	PRKCD	Protein kinase C, delta	60
NM-016457	PRKD2	Protein kinase D2	60
NM-021135	RPS6KA2	Ribosomal protein S6 kinase, 90 kDa, polypeptide 2	50
NM-005983	SKP2	S-phase kinase-associated protein 2 (p45)	61
NM-003318	TTK	TTK protein kinase	64
NM-031432	UCK1	Uridine-cytidine kinase 1	63
NM-006296	VRK2	Vaccinia-related kinase 2	55

^aApoptosis is at least 5% more than the control for both siRNA sequences of the kinase, as measured by HCS system, based on nuclear properties (morphology and Hoechst dye intensity) of the cells. Note: Growth inhibition resulted from siRNA silencing of SUM149 cells at 5 nM for 72 hours. Data shown here are the average results of both siRNA sequences of the kinase from two independent screens. For a complete list of active kinases identified in the siRNA library screen, refer to Additional file 1.

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ISSN: 1465-542X

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