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Figure 4 | Breast Cancer Research

Figure 4

From: MIBE acts as antagonist ligand of both estrogen receptor α and GPER in breast cancer cells

Figure 4

MIBE inhibits the transactivation of ERalpha induced by E2. (a) MCF7 cells were transfected with the ER luciferase reporter gene (EREluc) along with the internal transfection control Renilla Luciferase and treated with increasing concentrations (logarithmic scale) of E2, the ER antagonist OHT and MIBE. (b) MCF7 cells were transfected with the ER reporter gene and the internal transfection control Renilla Luciferase and treated with 10 nM E2 in combination with increasing concentration of OHT or MIBE, as indicated. (c, e) Hek293 cells were transfected with Gal4 reporter gene GK1, the Gal4 fusion proteins encoding the Ligand Binding Domain (LBD) of ERα (GalERalpha) or ERbeta (GalERbeta) and the internal transfection control Renilla Luciferase and treated with increasing concentrations (logarithmic scale) of E2, OHT and MIBE. (d, f) Hek293 cells were transfected with the Gal4 reporter gene GK1, the Gal4 fusion proteins GalERalpha or GalERbeta and the internal transfection control Renilla Luciferase and treated with 100 nM E2 in combination with increasing concentrations of OHT or MIBE, as indicated. Each data point represents the mean ± SD of three experiments performed in triplicate.

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