Hyperinsulinemia results in increased expression of c-Myc, MMP-9, IR-β, IGF-IR and VEGF in Mvt1 tumors. (A) Tumor tissue was extracted from control and MKR+/+ mice and subjected to SDS PAGE and Western blotting with antibodies to c-Myc, MMP-9, IR-β, IGF-IR and VEGF. β-actin was included as a loading control. (B) Fold change in expression of c-Myc, MMP-9, IR-β, IGF-IR and VEGF in control and MKR+/+ tumor tissue compared to β-actin control. *, P ≤ 0.05. (C) Semi-quantitative PCR showing relative mRNA expression levels of Igf1, Igf-2 and β-actin in tumor tissue from control and MKR+/+ mice. (D) Densitometric quantification of DNA gel electrophoresis showing mRNA expression of Igf1 and Igf2 relative to β-actin.