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Table 1 Estimation of tumor-initiating cell frequency of hypoxia-inducible factor-1α wild-type and knockout mammary tumor epithelial cellsa

From: Hypoxia-inducible factor 1α promotes primary tumor growth and tumor-initiating cell activity in breast cancer

 

MTEC genotype

   

Estimated cell frequency

HIF-1α WT tumor-positive

HIF-1α-KO tumor-negative

HIF-1α WT

HIF-1α-KO

P valuesb

Number of cells injected, % (n)

     

   500

78% (7 of 9)

0% (0 of 8)

  

0.0023

   200

90% (9 of 10)

10% (1 of 10)

  

0.0011

   100

77% (10 of 13)

9% (1 of 11)

  

0.0003

   50

69% (11 of 16)

6% (1 of 16)

  

0.0006

   25

30% (6 of 20)

0% (0 of 20)

  

0.0202

   10

35% (8 of 23)

0% (0 of 22)

  

0.0038

Estimated TIC frequency by ELDA (95% CI)

  

1 of 82 (1 of 57 to 1 of 117)

1 of 2,915 (1 of 925 to 1 of 9,193)

5.79e-21

  1. aELDA, Extreme Limiting Dilution Analysis; HIF-1α, hypoxia-inducible factor 1α; KO, knockout; MTEC, mammary tumor epithelial cell; TIC, tumor-infiltrating cell; WT, wild type. Frequency of tumors following limiting dilution transplantation of WT or KO MTECs is shown. The number of tumor-positive recipients per the total number of recipients at day 36 posttransplant and the P-values between genotypes for each cell density tested are listed. The estimated frequency of TICs was determined using the publicly available ELDA software. bP values were calculated using Fisher's exact test.