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Figure 8 | Breast Cancer Research

Figure 8

From: Hypoxia-inducible factor 1α promotes primary tumor growth and tumor-initiating cell activity in breast cancer

Figure 8

HIF-1α regulates tumor initiation potential in vivo. (A) Evaluation of the percentage of recipients (number of recipients positive for a palpable mass per number of recipients successfully transplanted × 100) that developed a tumor mass by day 36 postinjection of unsorted wild-type (WT) and knockout (KO) tumor cells. The asterisks indicate a significant difference between genotypes for a given cell density by Fisher's exact test. (B) Comparison of WT and KO mean tumor volumes ± SEM over time when 10, 25 or 50 cells were injected into the mammary fat pads. (C) The mean fold change ± SEM in gene expression profiles of early-stage tumor masses was evaluated by qRT-PCR. All genes displayed were downregulated in KO tumors as compared to WT tumors (n = 4 tumors/genotype/experiment).

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