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Figure 4 | Breast Cancer Research

Figure 4

From: Hypoxia-inducible factor 1α promotes primary tumor growth and tumor-initiating cell activity in breast cancer

Figure 4

Loss of cells expressing K14 or K5 in HIF-1α knockout tumors is accompanied by a reduction in the expression of markers of the epithelial-to-mesenchymal transition. (A) Frozen sections were prepared from tumors harvested from PyMT+ transgenic mice or from wild-type (WT) and knockout (KO) tumors harvested from FVB recipients. All tumors were ≥ 500 mm3 in volume. Sections were costained with antibodies to K14 (red) and K8 (green) or K5 (red) alone and counterstained with DAPI. Images were captured at ×200 original magnification. Scale bar = 50 μm. (B) The mean fold change ± SEM in gene expression observed in end-stage KO tumors of Krt14 (K14) and Krt5 (K5) mRNAs, as well as multiple markers of the epithelial-to-mesenchymal transition, as determined by qRT-PCR performed using the primers and probes listed in Additional file 1 Table S2 (n = 5 tumors/genotype). The mean ± SEM of biological replicates is graphed. The expression of Snail, Slug, Twist1, Fn1, Krt14 (K14) and Krt5 (K5) mRNAs was reduced in KO tumors compared to WT tumors; however, the expression of Krt18 (K18) varied by < 20% between genotypes.

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