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Figure 2 | Breast Cancer Research

Figure 2

From: Evidence that GTP-binding domain but not catalytic domain of transglutaminase 2 is essential for epithelial-to-mesenchymal transition in mammary epithelial cells

Figure 2

GTP-binding activity is critical for transglutaminase 2-induced epithelial-to-mesenchymal transition. (A) Phase-contrast images of MCF-10A cells expressing the lentiviral vector alone (Vec) or vector containing wild-type (TG2-WT) or mutant transglutaminase 2 (TG2) constructs (TG2-C277S and TG2-R580A) were taken after 48 hours of culture in puromycin selection medium. Original magnification ×200. (B) Immunoblot analysis of control (Vec) and TG2-expressing MCF10A cells. Expression of epithelial cell markers (E-cadherin and β-catenin) and mesenchymal cell markers (N-cadherin, fibronectin and vimentin) was examined by immunoblotting. (C) Immunofluorescence due to expression of epithelial-to-mesenchymal transition markers in MCF10A sublines. Cells were counterstained with 4',6-diamidino-2-phenylindole (blue) for nuclear staining.

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