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Table 1 Primers selected for confirmation of changes in the expression of tumor necrosis factor-α-related genes by reverse transcription quantitative polymerase chain reaction

From: Alterations in tumor necrosis factor signaling pathways are associated with cytotoxicity and resistance to taxanes: a study in isogenic resistant tumor cells

Gene Forward primer Reverse primer
S28 5'TCC ATC ATC CGC AAT GTA AAG-3' 5'-GCT TCT CGC TCT GAC TCC AAA-3'
TNFAIP3 5'-GAC CAT GGC ACA ACT CAT CTC A-3' 5'-GTT AGC TTC ATC CAA CTT TGC GGC ATT G-3'
TNFSF10 5'-CGT GTA CTT TAC CAA CGA GCT GA-3' 5'-ACG GAG TTG CCA CTT GAC TTG-3'
TNFSF13 5'-ACT CTC AGT TGC CCT CTG GTT G-3' 5'-GGA ACT CTG CTC CGG GAG ACT C-3'
TNFSF14 5'-TTT GCT CCA CAG TTG GCC TAA TC-3' 5'-CAA TGA CTG TGG CCT CAC CTT C-3'
TLR1 5'-GGT ACC AGG CCC TCT TCC TCG TTA G-3' 5'-TAG GAA CGT GGA TGA GAC CG TTT TT-3'
TLR6 5'-GCA AAA ACC CTT CAC CTT GTT TTT C-3' 5'-CCA AGT CGT TTC TAT GTG GTT GAG G-3'
BIRC3 5'-TGT TGG GAA TCT GGA GAT GA-3' 5'-CGG ATG AAC TCC TGT CCT TT-3'
TNFR1 5'-ACT GCC TCA GCT GCT CCA AAT-3' 5'-CCG GTC CAC TGT GCA AGA A-3'
TNFα 5'-TCT TCT CGA ACC CCG AGT GA-3' 5'-GGA GCT GCC CCT CAG CTT-3'
  1. Reaction conditions were as described in Materials and methods, and expression was assessed relative to that of S28, the internal reference gene.