Figure 4
Sensitivity of MCF-7 CC , MCF-7 TXT , and MCF-7 TAX-1 cells to tumor-necrosis factor (TNF)-α in clonogenic assays. The effect of docetaxel selection dose on colony formation in MCF-7CC and MCF-7TXT cells in the absence or presence of 10 ng/ml TNF (a). Colony formation in MCF-7, MCF-7TXT10, and MCF-7TAX-1 cells in the presence of 0 ng/ml, 10 ng/ml, 50 ng/ml, or 100 ng/ml TNF-α (b). The mean numbers of colonies (± standard error) are depicted, and the significance of differences in TNF-α sensitivity between MCF-7CC and MCF-7TXT cells at the various selection doses was assessed by using a Student t test; P values of < 0.05, < 0.01, and < 0.001 are represented by the *, **, and *** symbols, respectively.