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Figure 2 | Breast Cancer Research

Figure 2

From: A novel inhibitor of fatty acid synthase shows activity against HER2+ breast cancer xenografts and is active in anti-HER2 drug-resistant cell lines

Figure 2

G28UCM plus trastuzumab, lapatinib and erlotinib induced apoptosis in AU565 breast cancer cells. Induction of caspase activity was confirmed by PARP cleavage. AU565 cells were treated with G28UCM (30 μM) plus trastuzumab (1 μM), lapatinib (5 μM), erlotinib (8 μM) or cetuximab (15 μg/ml) for 12 and 24 h (G28UCM plus lapatinib or erlotinib), and 24 and 48 h (G28UCM plus trastuzumab or cetuximab), and equal amounts of lysates were immunoblotted with anti-PARP antibody which identified the 116 KDa (intact PARP) and the 89 KDa (cleavage product) bands. Blots were reprobed for β-actin as loading control. Gels shown are representative of those obtained from two or three independent experiments.

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Breast Cancer Research

ISSN: 1465-542X

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