Figure 3
From: Regulation of breast cancer metastasis by Runx2 and estrogen signaling: the role of SNAI2
SNAI2 is required for Runx2-mediated EMT. (A) RT-qPCR analysis of SNAI2 mRNA in MCF7/Rx2dox cells transduced with a control (sh-Neg) or each of two independent SNAI2 shRNA lentiviruses. (B-C), MCF7/Rx2dox cells transduced with sh1-SNAI2, sh2-SNAI2, or the negative control virus were treated with vehicle control (C), dox (D), E2 (E), or both stimulants (DE). The effects of SNAI2 knockdown on expression of the indicated EMT markers were assessed by RT-qPCR after seven days and the effects on invasion were assessed using Matrigelâ„¢-containing BD Biocoatâ„¢ Transwells as in Figure 2D, except dox treatment was initiated 6 hours prior to placing the cells in the transwell chambers. **, P < 0.01; ***, P < 0.005. Dox, doxycycline; EMT, epithelial-mesenchymal transition; E2, estradiol; RT-qPCR, reverse transcription - quantitative polymerase chain reaction; Runx2, runt related transcription factor 2; SNAI2, snail homolog.