Figure 1
From: Mammary Gland Cell Death Also Involves Lysosomal Autophagy
Histochemical Appearance of Involuting Mammary Glands. Cell death in the mammary gland was induced by weaning the pups from their nursing moms. Day 0 and day 4 mammary gland extracts were assayed for the presence of lysosomes using an acid phosphatase (AP) assay. Day 0 glands displayed faint AP staining (panels a and c). In contrast, by day 4 the glands showed intense AP-positive staining and the cytoplasm was highly vacuolated (panels b and d). Magnification: panels a and b, 250 X; panels c and d, 600 X.