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Figure 2 | Breast Cancer Research

Figure 2

From: Targeting cholesterol-rich microdomains to circumvent tamoxifen-resistant breast cancer

Figure 2

Methyl-β-cyclodextrin (MβCD), a known cholesterol-rich membrane domain disruptor, reduces levels of prosurvival mediators and, in combination with TAM, induces apoptosis and reduces levels of prosurvival mediators. (a) MCF-7/TAMR cells were treated with MβCD at 2.5 and 5.0 μM for 1 day. Protein levels of prosurvival signaling mediators were determined with Western blot analyses. (b) MCF-7/TAMR and MCF-7/HER-2 cells were treated with TAM at 0.5 or 1 μM with and without MβCD at 1.25 or 2.5 μM for 1 day. Apoptosis was determined with Annexin V/PI. (c) Western blot analyses were performed to determine the prosurvival signaling mediators in both TAMR cell lines treated separately and in combination with 1 μM TAM and 2.5 μM MβCD for 1 day. Data in (a) and (c) are representative of two individual experiments. Data in (b) are depicted as mean ± SD of three individual experiments. *Significantly different in comparison with TAM or MβCD treatment alone; P < 0.05. TAM, tamoxifen; TAMR, tamoxifen resistant; MCF-7/HER-2, clone 18 MCF-7 cells overexpressing HER-2; MCF-7/TAMR, acquired tamoxifen-resistant MCF-7.

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