Figure 1

Negative correlation of miRNA (miR)-34b expression level and estrogen receptor (ER) status in ER+ human breast cancers. (A) miR-34b expression in human breast cancer tissue. miR-34b expression levels were analyzed by quantitative PCR (qPCR) (ER+, n = 26; ER-, n = 21). The results are normalized to internal U6 snRNA expression. (B) Correlation analysis of ERα and miR-34b in ER+ human breast cancer tissues. P = 0.019. (C) Correlation analysis of ERα and miR-34b in ER- human breast cancer tissues. P = 0.968. For both parts (B) and (C), ERα mRNA expression levels were analyzed performing SYBR Green qPCR for each tissue sample. Data represent means ± SD of triplicate experiments (*P < 0.05). The results are normalized to internal TaqMan Low-Density Array expression.