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Figure 6 | Breast Cancer Research

Figure 6

From: ARTEMIN synergizes with TWIST1 to promote metastasis and poor survival outcome in patients with ER negative mammary carcinoma

Figure 6

ARTEMIN (ARTN) mediated oncogenicity is regulated by TWIST1 expression in estrogen receptor (ER) negative mammary carcinoma (ER-MC) cells. (a) Western blot analysis for TWIST1 in BT549 and MDA-MB-231 cells with forced expression of ARTN ± TWIST1 small interfering RNA (siRNA) showing efficiency of siRNA-mediated depletion of TWIST1 protein. Universal negative control was used as an internal control. (b) Monolayer proliferation assay (2% fetal bovine serum media). (c) Colony formation in soft agar: Cells were cultured in 0.35% agar for 10 days. Cell growth was measured using alamarBlue. (d) Cell growth (number) in 3D matrigel: cells were cultured in 3D matrigel for seven days. Cell growth was detected using alamarBlue. (e) Cell migration and (f) Cell invasion were determined by use of transwell inserts as described in materials and methods. siRNA control-treated vector (VEC) cells presented as 100%. *, P < 0.05; **, P < 0.01.

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Breast Cancer Research

ISSN: 1465-542X

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