Figure 2

ARTEMIN (ARTN) modulates cellular oncogenicity and motility of estrogen receptor (ER) negative mammary carcinoma (ER-MC) cells. (a) Cell growth (number) in 3D matrigel. BT549-ARTN and MDA-MB-231-ARTN cells and their respective vector (VEC) cells were cultured in 3D matrigel for seven days. Cell growth was measured using alamarBlue. (b) Cell migration, and (c) Cell invasion for BT549-ARTN and MDA-MB-231-ARTN cells and their respective VEC cells were determined by use of transwell inserts as described in materials and methods. Respective VEC cells are presented as 100%. (d) Cell growth (number) in 3D matrigel. BT549-siARTN and MDA-MB-231-siARTN cells and their respective control (siCONT) cells were cultured in 3D matrigel for seven days. Cell growth was measured using alamarBlue. (e) Cell migration, and (f) Cell invasion for BT549-siARTN and MDA-MB-231-siARTN cells and their respective siCONT cells were determined by use of transwell inserts as described in materials and methods. Respective siCONT cells are presented as 100%. **, P < 0.01; ***, P < 0.001.