Figure 1

ARTEMIN (ARTN) modulates cellular morphology of estrogen receptor (ER) negative mammary carcinoma (ER-MC) cells. (a) Monolayer adherent morphology of BT549 and MDA-MB-231 cells with forced expression or depletion of ARTN. Cultures were imaged at approximately 40% confluence and under 400 × magnification (Bar represents 20 μm). (b) BT549-ARTN and MDA-MB-231-ARTN cells with their respective vector (VEC) cells and BT549-siARTN and MDA-MB-231-siARTN with their control (siCONT) cells were seeded at a very low cell density (1000 cell plated in 10 cm petri dish). Colonies formed by each cell types, were categorized and counted as per the extent of colony scattering. Percentages of each colony category in the total counts were plotted as indicated. (c) ARTN modulates organization of filamentous actin as visualised by fluorescein isothiocyanate (FITC) - phalloidin in BT549 and MDA-MB-231 cells with forced expression or depletion of ARTN compared with the respective control cells. Bar, 50 μm (f-actin staining for BT549-ARTN cells), 62.7 μm (f-actin staining for MDA-MB-231-ARTN cells). *, P < 0.05; **, P < 0.01.