Figure 3
SQUID detection of nanoparticle binding versus time. (a) Cells (7.5 × 106) from each breast cancer cell line or media alone were incubated with 0.8 mg of nanoparticles for 15 minutes on ice. SQUID measurements were taken every 2 minutes. The curves represent non-linear fits of the data. Data represent results from two separate experiments, and error bars represent the standard deviation. (b) Photomicrographs show 40× imaging of Prussian blue histochemical staining for the presence of iron oxide nanoparticles on cells incubated with anti-Her2 antibody-conjugated nanoparticles. Scale bars = 20 μM. Her2, human epidermal growth factor-like receptor 2; SQUID, Superconducting Quantum Interference Device.