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Figure 2 | Breast Cancer Research

Figure 2

From: Metastatic breast cancer cells inhibit osteoblast differentiation through the Runx2/CBFβ-dependent expression of the Wnt antagonist, sclerostin

Figure 2

Runx2 and CBFβ are required for the expression of genes encoding the soluble ligands, IL-11, sclerostin and GM-CSF in MDA-MB-231 cells. (A) Venn diagram showing the number of genes with ≥ 2-fold expression-change in each microarray. (B) Validation of Runx2 and CBFβ target genes. MDA-MB-231 cells transfected with siRunx2, siCBFβ or a non-specific siRNA were subject to real-time qRT-PCR. Acidic ribosomal phosphoprotein P0 (RPLO) mRNA was used as a control for normalization and relative values are shown. Data are presented as mean ± standard deviation (SD) (n = 3). Statistical evaluation of significant differences was performed using the Student's t-test. Asterisk (*) indicates P < 0.05 when compared to control (siNS).

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