Runx2 and CBFβ are required for the expression of genes encoding the soluble ligands, IL-11, sclerostin and GM-CSF in MDA-MB-231 cells. (A) Venn diagram showing the number of genes with ≥ 2-fold expression-change in each microarray. (B) Validation of Runx2 and CBFβ target genes. MDA-MB-231 cells transfected with siRunx2, siCBFβ or a non-specific siRNA were subject to real-time qRT-PCR. Acidic ribosomal phosphoprotein P0 (RPLO) mRNA was used as a control for normalization and relative values are shown. Data are presented as mean ± standard deviation (SD) (n = 3). Statistical evaluation of significant differences was performed using the Student's t-test. Asterisk (*) indicates P < 0.05 when compared to control (siNS).