Figure 5

NRC-03 and NRC-07 enter breast cancer cells and localize to the nucleus. MDA-MB-231 breast cancer cells were cultured in the presence or absence of 50 μM biotinylated-NRC-03, or -NRC-07 for 30 seconds. (a) The actin cytoskeleton and biotinylated-peptides were visualized with confocal microscopy (×1,000) by using Alexa-Fluor 488-phalloidin and Texas Red-conjugated streptavidin, respectively. (b) The nucleus and biotinylated-peptides were visualized with confocal microscopy (×1,000) by using TO-PRO-3 iodide and Texas Red-conjugated streptavidin, respectively. Images shown are from a representative experiment (n = 3).