Figure 4

NRC-03 and NRC-07 damage the cell membrane of breast cancer cells but not fibroblasts. (a) MDA-MB-231 breast cancer cells or (b) normal dermal fibroblasts were cultured in the absence or presence of 50 μM NRC-03 or NRC-07 for 30 minutes. Membrane damage was visualized with scanning electron microscopy. Data shown are from a representative experiment (n = 2). (c) MDA-MB-231 cells were cultured in the absence or presence of the indicated concentrations of NRC-03 and NRC-07. Cytotoxicity was measured with the LDH-release assay after 10 minutes, 30 minutes, and 1, 4, and 8 hours. Data are expressed as the mean ± SEM of three independent experiments.