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Figure 5 | Breast Cancer Research

Figure 5

From: Hsp27 participates in the maintenance of breast cancer stem cells through regulation of epithelial-mesenchymal transition and nuclear factor-κB

Figure 5

Inhibition of Hsp27 suppressed EMT signature of BCSCs. (A) Cell migration ability of AS-B145, ALDH+ AS-B244, MDA-MB-231 or Sca-1+ 4T1 cells in present of quercetin (Q, 12.5, 25 or 50 μM) or 0.1% DMSO or was analyzed with wound-healing assay. *, P < 0.05; #, P < 0.01. (B) Cell migration ability of AS-B145, MDA-MB-231 or ALDH+ AS-B244 cells after transfection of Hsp27 siRNA (si-Hsp27) or negative control siRNA (ctrl siRNA) was analyzed with wound-healing assay. *, P < 0.05; #, P < 0.01. (C) EMT related proteins (E-cadherin, vimentin or snail) of AS-B145 or ALDH+ AS-B244 cells were analyzed by Western blot. Inserted values indicated relative expression of proteins in negative control siRNA (ctrl) or si-Hsp27 (KD) transfected cells. ALDH: aldehyde dehydrogenase; BCSCs: breast cancer stem cells; EMT, epithelial-mesenchymal transition.

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