Figure 1

Increased expression of Hsp27 in ALDH+ BCSCs. (A) ALDH- or ALDH+ cells were sorted from BC0145 and BC0244 xenografts and 150 μg of extracted total protein were subjected into PVDF membrane of MAPK array as described in "Materials and methods" section. P-Hsp27, phosphorylated Hsp27; p-p38 MAPK, phosphorylated p38 MAPK. (B) ALDH- or ALDH+ cells were sorted from AS-B244 cells and 25 μg of total protein were used for Hsp27 or GAPDH detection by Western blot. Inserted values indicated relative expression of Hsp27 in ALDH- or ALDH+ cells. ALDH, aldehyde dehydrogenase; MAPK, mitogen activated protein kinase.