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Table 1 PCR and DHPLC conditions for the mutational analysis of E-cadherin

From: Low frequency of E-cadherinalterations in familial breast cancer

  Annealing Amplicon DHPLC
  temperature length temperature
Exon no (°C) (bp) (°C)
1 70 285 66
2 55 377 62
3 60 352 58, 61
4 60 351 60
5 60 398 58
6 58 246 59
7 60 329 60, 62, 64
8 58 225 59, 62
9 55 252 59
10 55 309 58
11 60 243 61
12 60 326 59, 61
13 58 297 58
14 60 206 61
15 60 244 58
16 60 318 59