Table 1 Antibodies and their dilutions used in immunohistochemistry
Target | Clone | Dilution | Manufacturer |
|---|---|---|---|
CK5 | M7237 | 1:25 | DakoCytomation, Glostrup, Denmark |
CK18 | NCL-CK18 | 1:20 | Novocastra Laboratories Ltd, UK |
COX2 | 160122 | 1:200 | Cayman Chemical, Ann Arbor, MI, USA |
E-cadherin | #4065 | 1:200 | Cell Signaling Technology Inc, Danvers, MA, USA |
EGFR | NCL-EGFR | 1:500 | Novocastra Laboratories Ltd |
ER | 6F11 | 1:500 | Novocastra Laboratories Ltd |
GATA3 | HG3-31 | 1:300 | Santa Cruz Biotechnology Inc, Santa Cruz, CA, USA |
HER2 | CB11 | 1:200 | Novocastra Laboratories Ltd |
Ki-67 | MM-1 | 1:1000 | Novocastra Laboratories Ltd |
KIT | A4502 | 1:300 | DakoCytomation |
Nestin | MAB5326 | 1:400 | Chemicon International Inc, Temecula, CA, USA |
p53 | D07 | 1:500 | Novocastra Laboratories Ltd |
PgR | 312 | 1:500 | Novocastra Laboratories Ltd |
Prominin | AC133 | 1:10 | Miltenyi Biotec GmbH, Germany |
SMA | M0581 | 1:100 | DakoCytomation |
SNAI1 | ARP33314 | 1:400 | Aviva Systems Biology, San Diego, CA, USA |
SNAI2 | D-19 | 1:100 | Santa Cruz Biotechnology Inc. |