Figure 3

MAP-tau expression and frequency distribution in TAX 307S whole tissue sections. (a) Whole section breast tumor tissue from patient case 24 stained with hematoxylin and eosin showing dark pink staining of tumor cytoplasm compared with blue nuclei staining (red arrows); remaining light pink or non-staining, mesh-like areas comprised of breast adipose tissue (black arrow). Original magnification × 4. (b) Whole tissue slide of breast tumor tissue from panel a (case 24) stained with MAP-tau-Cy5 and analyzed using AQUA digital pathology algorithms. A matrix captured 103 quadrants (41 quadrants displayed in panel b and was used to systematically capture each field of view (FOV), creating 103 unique expression scores for case 24. Original magnification × 4. (c) Frequency distribution for MAP-tau quadrant scores from case 24 with mean and median AQUA scores of 1357 and 1369, respectively, and a score range of 610 to 1986. FOVs were used to generate a frequency distribution for each case in the TAX 307S cohort (n = 140). The median AQUA score from each case was used in all cohort analyses. (d) Distribution of MAP-tau expression in TAX307.