Figure 1

MAP-tau expression and frequency distribution in Yale University cohort with two-fold redundancy. (a) Representative case from the Yale University cohort TMA showing cytoplasmic localization of MAP-tau within breast tumor tissue (two-fold redundancy; n = 651). (i) Pixel binary gating demarcating epithelial tumor area from stroma in order to define the tumor compartment; (ii) immunofluorescence of MAP-tau-Cy5 (red) expression pattern (continuous score of 789); (iii) target protein MAP-tau-Cy5 (red) colocalization with cytokeratin-Cy3, a marker for breast epithelia (green); (iv) Localization of nuclear DAPI (blue) relative to colocalization of target protein MAP-tau-Cy5 (red) with cytokeratin-Cy3, a marker for breast epithelia (green). Original magnification x20. (b) Yale frequency distribution of average MAP-tau expression scores in the Yale University cohort with preselected cutpoint 462, the median MAP-tau expression score observed in normal breast tissue and used to differentiate high expressors from low expressors.