A reduction in tumor cell seeding is observed in lungs from IKFM mice. IKFM and control mice were treated with doxycycline (dox; 2 g/L) for one week before injection of PyVT R221A cells. Lungs were harvested at (a) one hour (n = 5 control, n = 7 IKFM, P = 0.4410) and (b) six hours (n = 3 control, n = 3 IKFM, P = 0.0214) post injection and RNA isolated from whole lung samples for quantitative real-time PCR analysis of Polyoma (PYL) expression. (b) Western blot for cleaved caspase-3 on whole cell lung homogenates from mice treated with dox for one week (no cells injected; n = 4 control and n = 4 IKFM) and homogenates from the six hour time point (n = 4 control and n = 4 IKFM). (c) IKFM mice and controls were treated with dox for one week and then imaged for ROS (n = 3 control and n = 3 IKFM; P = 0.0011). (e) Real-time PCR analysis of whole lung RNA for CXCL9 (n = 4 control, n = 3 IKFM, P = 0.0033).