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Figure 4 | Breast Cancer Research

Figure 4

From: Septin 9 isoform expression, localization and epigenetic changes during human and mouse breast cancer progression

Figure 4

Hypermethylation of the SEPT9_v3 promoter occurs in human breast primary carcinomas. (A) Cell lines were treated with 5-aza-2'-deoxycytidine, and SEPT9_v3 mRNA was quantified. Representative cell lines with hypermethylation (left graph) and hypomethylation (right graph) of the SEPT9_v3 promoter region are displayed. U: untreated cells; T: treated cells. (B) SEPT9_v3 promoter region activity. Top: Location of tested luciferase fragments relative to the DMR. Bottom: Quantification of these fragments via luciferase reporter assay. (C) Supervised clustering of MassARRAY DNA methylation profiles in human primary adenocarcinomas, matching tumor-free areas and normal breast tissues. Right graph: Average levels of DNA methylation between tumors and tumor-free matching samples show a statistically significant difference.

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Breast Cancer Research

ISSN: 1465-542X

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